Neuroprotective Effects of Gagam-Sipjeondaebo-Tang, a Novel Herbal Formula, against MPTP-Induced Parkinsonian Mice and MPP⁺-Induced Cell Death in SH-SY5Y Cells

Figure 5

Effects of GST on the Akt and ERK signaling in SH-SY5Y cells. (a) Akt and ERK activation by GST. SH-SY5Y cells were treated with 100 μg/ml GST for the indicated times. The protein levels of Akt and ERK and their phosphorylated forms were analyzed by western blot. (b) Role of Akt and ERK activation by GST in mitochondrial function. After LY294002 and PD98059 pretreatment (10 μM, 1 h), cells were incubated with GST for 1 h and then treated with 1 mM MPP⁺ for 18 h. After staining with rhodamine 123, cells were subjected on flow cytometer. ### p < 0.001 compared to control group, ∗∗∗ p < 0.001 compared to MPP⁺ treatment, &&& p < 0.001 compared to MPP⁺+GST treatment, analyzed by one-way ANOVA, followed by Bonferroni’s post hoc test.

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