Effects of Angelica gigas Nakai as an Anti-Inflammatory Agent in In Vitro and In Vivo Atopic Dermatitis Models
Effect of AGNE on histamine, IL-1β, IL-4, IL-6, IL-8, IL-10, and TNF-α level in PMACI-stimulated HMC-1 cells. (a) Cells (6 × 105 cells/well) were pretreated with AGNE (0.1, 1, and 10 μg/mL) for 1 h and then PMACI (50 μM PMA + 1 μg/mL CI A23187) for 4 h. Histamine content was measured using a histamine assay kit. (b–g) Cells were pretreated with AGNE for 1 h and then PMACI for 12 h. Levels of inflammatory cytokines were measured using ELISA. Data is mean ± SEM of triplicate determinations from separate triplicate experiments ( versus control and versus PMACI alone).