Ethyl Acetate Fraction from Hedyotis diffusa plus Scutellaria barbata Exerts Anti-Inflammatory Effects by Regulating miR-155 Expression and JNK Signaling Pathway
Table 1
Screening extracts and fractions from HDSB on NO production .
Groups
Extracts/fractions
Dose (μg/ml)
Nitrite (μM)
Inhibition (%)
IC50 (μg/ml)
Yield (%)
Control
-
-
4.12 ± 0.01
Model
-
-
53.80 ± 0.02
1 : 1 W
Water extract
10
51.79 ± 0.04
4.04
NC
10.45
50
50.33 ± 0.02
6.98
100
50.64 ± 0.02
6.35
200
52.61 ± 0.03
2.39
Petroleum ether fraction
10
49.45 ± 0.02
8.75
52.61
0.1
50
36.75 ± 0.03
34.31
100
21.14 ± 0.02
65.72
200
6.87 ± 0.01
94.46
Ethyl acetate fraction (EA11)
10
48.10 ± 0.03
11.46
27.74
0.58
50
18.09 ± 0.02
71.87
100
5.98 ± 0.00
96.24
200
5.96 ± 0.01
96.28
-Butanol fraction
10
48.79 ± 0.03
10.08
177
6.13
50
40.80 ± 0.03
26.17
100
34.00 ± 0.02
39.86
200
27.63 ± 0.01
52.67
Water fraction
10
54.26 ± 0.02
0
685.57
30.98
50
52.10 ± 0.04
3.43
100
50.45 ± 0.03
6.73
200
53.02 ± 0.02
1.57
1 : 2 W
water extract
10
51.00 ± 0.03
5.63
NC
10.61
50
46.34 ± 0.02
15
100
48.44 ± 0.01
10.79
200
45.29 ± 0.01
17.12
petroleum ether fraction
10
52.35 ± 0.01
2.91
128.41
0.8
50
45.19 ± 0.06
17.33
100
32.75 ± 0.02
42.37
200
20.44 ± 0.02
67.14
ethyl acetate fraction
10
49.67 ± 0.04
8.3
30.2
2.11
50
17.02 ± 0.02
74.02
100
6.37 ± 0.00
95.46
200
5.75 ± 0.00
96.71
n-butanol fraction
10
50.93 ± 0.02
5.77
97.09
3.81
50
33.60 ± 0.01
40.65
100
28.87 ± 0.01
50.18
200
21.20 ± 0.01
65.61
water fraction
10
57.06 ± 0.02
0
456.72
58.34
50
50.48 ± 0.00
6.67
100
52.44 ± 0.02
2.73
200
50.90 ± 0.02
5.83
2 : 1 W
water extract
10
50.91 ± 0.03
5.82
NC
7.23
50
49.81 ± 0.03
8.03
100
53.67 ± 0.02
0.25
200
53.00 ± 0.02
1.6
petroleum ether fraction
10
52.93 ± 0.03
1.75
199.67
0.03
50
45.09 ± 0.04
17.53
100
40.15 ± 0.07
27.47
200
30.07 ± 0.05
47.76
ethyl acetate fraction
10
50.76 ± 0.01
6.11
44.8
1.53
50
33.62 ± 0.01
40.62
100
19.55 ± 0.02
68.93
200
4.90 ± 0.01
98.42
-butanol fraction
10
50.35 ± 0.02
6.93
661.8
4.62
50
47.54 ± 0.03
12.6
100
40.98 ± 0.03
25.8
200
37.73 ± 0.02
32.34
water fraction
10
53.68 ± 0.04
0.24
1582.6
35.97
50
51.54 ± 0.02
4.54
100
50.65 ± 0.03
6.33
200
51.38 ± 0.03
4.88
L-NIL
50 (μM)
39.53 ± 0.02
35.2
Values are presented as the mean ± standard deviation from three replicates. C, vehicle control; M, model group (LPS/IFNγ stimulation); EA11, ethyl acetate fraction from 1 : 1 W; vs. C group; vs. M group; vs. M group.
