Modulation of Neuronal Activity on Intercalated Neurons of Amygdala Might Underlie Anxiolytic Activity of a Standardized Extract of<i> Centella asiatica</i> ECa233

<div>Ionotropic glutamate receptors mediating electrically evoked EPSCs (eEPSCs) recorded in the ITCs. Voltage clamp recording after ITC stimulation at <svg height="11.8174pt" id="M30" style="vertical-align:-3.1815pt" version="1.1" viewbox="-0.0498162 -8.6359 15.6938 11.8174" width="15.6938pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M697 650H468L461 623L492 619C539 613 547 605 518 546C481 471 367 264 278 116H276C239 278 197 500 186 567C180 604 185 613 226 619L252 623L260 650H24L17 623C78 617 92 613 108 533L216 -11H247C365 200 515 462 560 529C616 612 624 615 689 623L697 650Z" id="g113-87"></path></g><g transform="matrix(.0091,0,0,-0.0091,7.332,3.132)"><path d="M870 650H600L593 618C681 609 684 605 669 516L641 367H261L286 516C302 603 313 610 391 618L398 650H132L123 618C214 610 218 606 200 516L127 130C113 46 107 40 25 32L18 0H290L298 32C204 40 200 42 214 130L250 321H631L596 131C580 47 573 39 484 32L475 0H753L762 32C673 39 668 46 685 130L757 516C772 606 778 610 861 618L870 650Z" id="g50-73"></path></g></svg> (voltage holding) ranging from −70 mV to +50 mV. (a) Summary plot of the peak amplitude of glutamate, NMDA, and nonglutamate receptor-mediated eEPSCs against <span class="nowrap"><svg height="11.8174pt" id="M31" style="vertical-align:-3.1815pt" version="1.1" viewbox="-0.0498162 -8.6359 15.6938 11.8174" width="15.6938pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M697 650H468L461 623L492 619C539 613 547 605 518 546C481 471 367 264 278 116H276C239 278 197 500 186 567C180 604 185 613 226 619L252 623L260 650H24L17 623C78 617 92 613 108 533L216 -11H247C365 200 515 462 560 529C616 612 624 615 689 623L697 650Z" id="g113-87"></path></g><g transform="matrix(.0091,0,0,-0.0091,7.332,3.132)"><path d="M870 650H600L593 618C681 609 684 605 669 516L641 367H261L286 516C302 603 313 610 391 618L398 650H132L123 618C214 610 218 606 200 516L127 130C113 46 107 40 25 32L18 0H290L298 32C204 40 200 42 214 130L250 321H631L596 131C580 47 573 39 484 32L475 0H753L762 32C673 39 668 46 685 130L757 516C772 606 778 610 861 618L870 650Z" id="g50-73"></path></g></svg>.</span> (b) Glutamate receptor-mediated synaptic currents in the presence of bicuculline (10 <i>μ</i>M). (c) NMDA receptor-mediated eEPSCs in the presence of bicuculline (10 <i>μ</i>M) and CNQX (50 <i>μ</i>M). (d) Nonglutamate receptor-mediated eEPSCs in the presence of bicuculline (10 <i>μ</i>M), CNQX (50 <i>μ</i>M), and APV (10 <i>μ</i>M).</div>

Evidence-Based Complementary and Alternative Medicine

fig4

Figure 4

Figure 4: Modulation of Neuronal Activity on Intercalated Neurons of Amygdala Might Underlie Anxiolytic Activity of a Standardized Extract of<i> Centella asiatica</i> ECa233 

Figure 4 | Modulation of Neuronal Activity on Intercalated Neurons of Amygdala Might Underlie Anxiolytic Activity of a Standardized Extract of Centella asiatica ECa233 