Research Article
Src Is a Prime Target Inhibited by Celtis choseniana Methanol Extract in Its Anti-Inflammatory Action
Table 1
Instrument and working conditions for quercetin, luteolin, and kaempferol analysis by high-performance liquid chromatography (HPLC).
| Instrument | KNAUER Corp. HPLC system |
| Column | Phenomenex, Gemini 5 μ C18 110A, 250 × 4.60 mm 5 μm | Detector | UV/VIS detector (370 nm) | Solvent A | 0.1% TFA in H2O | Solvent B | 0.08% TFA in 95% MeCN + 5% H2O | Standard | Dilution with DMSO | Sample treatment | 50 mg/mL dilution with DMSO | Injection volume | 20 μL | Flow rate | 1.0 mL/min | Gradient elution system | % A | % B | Time (min) | | | 0 | 100 | 0 | 50 | 50 | 50 | 60 | 0 | 100 |
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