Research Article

Src Is a Prime Target Inhibited by Celtis choseniana Methanol Extract in Its Anti-Inflammatory Action

Table 1

Instrument and working conditions for quercetin, luteolin, and kaempferol analysis by high-performance liquid chromatography (HPLC).

InstrumentKNAUER Corp. HPLC system

ColumnPhenomenex, Gemini 5 μ C18 110A, 250 × 4.60 mm 5 μm
DetectorUV/VIS detector (370 nm)
Solvent A0.1% TFA in H2O
Solvent B0.08% TFA in 95% MeCN + 5% H2O
StandardDilution with DMSO
Sample treatment50 mg/mL dilution with DMSO
Injection volume20 μL
Flow rate1.0 mL/min
Gradient elution system% A% B
Time (min)
 01000
 505050
 600100