Induction of Apoptosis and Inhibition of Epithelial Mesenchymal Transition by α-Mangostin in MG-63 Cell Lines

Figure 3

Mitochondrial membrane permeability was increased by α-mangostin treatment in MG-63 cells. Cells were treated with α-mangostin (0, 10, 25, and 50 μM) for 24 h. (a and b) Bcl-2 and Bak protein expressions exposed with α-mangostin were examined by western blot. The β-actin is a loading control. (c) MMP reduction was measured by JC-1 staining using FACS analysis. (d) Translocation of cytochrome c from mitochondria into nucleus was observed by a laser confocal fluorescence microscope. versus untreated cells (control).

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