Pharmacokinetics and Tissue Distribution Study of Pinosylvin in Rats by Ultra-High-Performance Liquid Chromatography Coupled with Linear Trap Quadrupole Orbitrap Mass Spectrometry

Figure 2

Representative selected ion monitoring (SIM) chromatograms of blank plasma (a), blank urine (b), blank feces (c), blank heart (d), blank liver (e), blank spleen (f), blank lung (g), blank kidney (h), blank large intestine (i), blank small intestine (j), blank stomach (k), plasma spiked with pinosylvin and isoliquiritigenin solution (IS) (l), urine spiked with pinosylvin and IS (m), feces spiked with pinosylvin and IS (n), stomach spiked with pinosylvin and IS (o), liver spiked with pinosylvin and IS (p), kidney spiked with pinosylvin and IS (q), plasma sample (r) obtained 10 min after oral administration of 49.44 mg/kg pinosylvin, urine sample (s) obtained 0–2 h after oral administration of 49.44 mg/kg pinosylvin, feces sample (t) obtained 4–6 h after oral administration of 49.44 mg/kg pinosylvin, and heart sample (u), liver sample (v), spleen sample (w), lung sample (x), kidney sample (y), large intestine sample (z), small intestine sample (aa), stomach sample (ab) obtained 10 min after oral administration of 49.44 mg/kg pinosylvin. Peak 1 reflects IS, and peak 2 reflects pinosylvin.

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