Pharmacokinetics and Tissue Distribution Study of Pinosylvin in Rats by Ultra-High-Performance Liquid Chromatography Coupled with Linear Trap Quadrupole Orbitrap Mass Spectrometry

Table 3

Extraction recovery and matrix effect of pinosylvin in rat plasma, urine, feces, and various tissue homogenates (n=5).

Bio-sample	Nominal concentrations (ng/mL)	Extraction recovery (%)	RSD (%)	Matrix effect (%)	RSD (%)
Plasma	5.4	98.8±7.1	8	99.1±8.5	8.6
	135	95.2±2.5	3	103.3±5.4	5.8
	540	99.5±3.7	4.2	114.8±3.3	3.2
Urine	1350	91.6±8.0	9.8	110.3±10.6	10.8
	5400	108.5±2.5	2.6	81.3±5.3	7.3
	54000	98.3±6.8	7.7	107.2±9.2	9.6
Feces	270	83.3±7.3	9.7	107.8±7.7	8
	2700	99±6.1	7	98.2±6.7	7.6
	5400	96.4±4.4	5.1	97.8±5.9	6.8
Heart	540	89.8±5.1	6.3	103.7±6.8	7.3
	5400	99.3±5.3	6	94.1±7.1	8.4
	27000	99.8±2.6	2.9	96.9±6.5	7.5
Liver	2700	86.5±4.8	6.3	103.2±4.7	5.1
	13500	83.4±5.5	7.4	88.4±7.9	10
	54000	87.1±3.8	4.9	89.3±5.5	6.9
Spleen	270	91.1±7.3	9	89.7±7.9	9.8
	5400	93.5±7.3	8.7	94.8±7.3	8.7
	13500	93.7±5.2	6.2	93.7±8	9.6
Lung	540	98.4±6.4	7.3	88.5±5.3	6.8
	2700	91.5±6.7	8.2	91.6±7.8	9.4
	13500	86±4	5.2	90.5±6.9	8.5
Kidney	540	86.5±6.1	7	98.4±8	8.2
	5400	99.6±4.8	4.9	101.8±6.2	6.1
	13500	90.5±6.4	7	92.6±9.2	9.9
Large intestine	2700	96.5±8.3	9.6	107.1±9.4	9.8
	13500	98.6±5	5.7	99±6.8	7.7
	54000	98.9±5	5.6	93.0±7.6	9.1
Small intestine	5400	89.4±10.7	13.3	88.7±7.7	9.7
	108000	88.6±6	7.5	89.4±8	10
	216000	92.9±7.5	9.1	83.2±1.3	1.7
Stomach	13500	87.5±10.1	13	90.8±7.1	8.8
	216000	89.9±7.7	9.6	94±11.4	13.5
	864000	86.3±9.2	12	92.6±9	10.8