Renoprotective Effect of Yiqi Yangyin Huayu Tongluo Formula against Diabetic Nephropathy in Diabetic Rats

Figure 5

ZY protected kidney of diabetic rats from apoptosis. ZY formula protected kidney of diabetic rats from apoptosis. (a) The level of renal cells apoptosis was measured by flow cytometry assay and the data were expressed as mean ±SD. p<0.05, p<0.01. (b) The mRNA level of caspase-3 was examined by real-time PCR. The data were expressed as mean ±SD. p<0.05, p<0.01. (c) The expression of caspase-3 in the kidney was examined by immunohistochemically staining (400× magnification). (d) Western blot was used to examine the expression of caspase-3 and cleaved caspase-3. The Western blot results were presented on the left and the semiquantitative results determined by WB were presented on the right. p<0.05, p<0.01. SD rats were divided into normal group, model group, Irbesartan group, and ZY group.