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Evidence-Based Complementary and Alternative Medicine
Volume 2018, Article ID 4280343, 9 pages
Research Article

A New Model of Diarrhea with Spleen-Kidney Yang Deficiency Syndrome

1Department of Gastroenterology, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China
2Department of Gastroenterology, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China
3Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
4Basic Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China
5Traditional Chinese Medicine Department of Gastroenterology, China-Japan Friendship Hospital, Beijing 100029, China
6College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
7Division of Gastroenterology and Hepatology, Johns Hopkins University, School of Medicine, Baltimore, MD 21224, USA

Correspondence should be addressed to Jiande JD Chen; moc.liamg@nehczdednaij and Wei Wei; moc.anis@yytxxs

Received 29 November 2017; Revised 2 March 2018; Accepted 20 March 2018; Published 30 September 2018

Academic Editor: Xiu-Min Li

Copyright © 2018 Jiajie Zhu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. The aim of this study was to examine a new method to create a rat model of diarrhea with spleen-kidney yang deficiency syndrome. Methods. A senna leaf (Folium sennae) decoction was made in 3 concentrations of 1.0, 0.5, and 0.25 g/mL. Rats were randomly divided into 4 groups: the control (C)-, high (H)-, middle (M)-, and low (L)- dose groups. The groups received saline, 1.0, 0.5, or 0.25 g/mL senna leaf decoction, respectively, for 4 weeks. Body weight monitoring, food consumption, water intake, defecation frequency, stool Bristol score, weight-loaded forced swimming test, forelimb grip strength test, D-xylose absorption test, serum cortisone, adrenocorticotropic hormone (ACTH), 24 h urine 17-hydroxycorticosteroid (17-OHCS), and histopathological detection were conducted to assess the success of the senna leaf decoction-induced model. Results. This study showed that the senna leaf decoction could induce diarrhea and dose-dependently slow body weight growth, reduce food consumption, and increase water intake, stool Bristol score, and defecation frequency. Statistical differences were found between groups H and M in rectal temperature, weight-loaded forced swimming time, forelimb grip strength, and serum cortisone. The D-xylose absorption test also showed dysfunction of intestinal absorption in groups H and M. The serum cortisone and 24 h urine 17-OHCS were significantly reduced in group H. Conclusions. Gastric gavage of 10 mL/kg of body weight of a high concentration of a senna leaf decoction (1.0 g/mL) for 4 weeks was used to create a rat model of diarrhea with spleen-kidney yang deficiency syndrome.