Research Article

Electrochemically Reduced Water Delays Mammary Tumors Growth in Mice and Inhibits Breast Cancer Cells Survival In Vitro

Figure 6

Effect of ERW on apoptosis in breast cancer cell lines. (a) The expression of Bax, Bcl-2, p53, and PARP-1 was assessed by Western blotting analysis in breast cancer cell lines that were treated with ERW and autoclaved ERW for 48 hours. Actin was used as an internal control. (b) The densitometric ratios between Bax and Bcl-2, p53 and actin, PARP-1 and actin, and statistical analysis are reported. Data are expressed as the mean ± SD of two independent experiments. The statistical significance of the effects obtained with ERW was calculated versus those obtained with autoclaved ERW (p ≤ 0.05, p ≤ 0.01).
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