(+)-Usnic Acid Inhibits Migration of c-KIT Positive Cells in Human Colorectal Cancer

Figure 8

8 M of (+)-UA induced transcriptional inhibition of c-KIT. (a) Western blot: the treatments of HCT116 cells with (+)-UA (8 μM, 48 hours) or (+)-UA (8 μM, 48 hours) + SCF (100 ng/ml, 30 minutes) induced UBC9 upregulation, thereby mediating the increase in SUMO-TFAP2A, which ultimately resulted in the downregulation of c-KIT, which were reversed by silencing UBC9. (b) Quantitative analysis: , , , , , , , , and , versus scramble RNA; , , and , versus SCF + (+)-UA. (c-d) RT-qPCR: , , and , versus scrambled siRNA; , versus SCF + (+)-UA.

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