1-(5-Bromo-2-hydroxy-4-methoxyphenyl)ethanone [SE1] Inhibits MMP-9 Expression by Regulating NF-B and MAPKs Signaling Pathways in HT1080 Human Fibrosarcoma Cells

Figure 3

Effects of SE1 on activation and expression of MMP-9 and MMP-2 in HT1080 cells. (a) Gelatin zymography for the determination of MMP-2 and MMP-9 activities in SE1-treated HT1080 cells. HT1080 cells treated with SE1 (10, 20, 50, and 100 μM) for 1 h and stimulated by PMA (10 ng mL⁻¹) for 72 h. Gelatinolytic activities of MMP-2 and MMP-9 in conditioned media were detected by electrophoresis of soluble protein on a gelatin containing 10% polyacrylamide gel. Untreated control was used as a loading control. (b) Expression of MMP-2 and MMP-9 in cell lysates was detected using western blot analysis. β-actin was used as a loading control. HT1080 cells treated with SE1 (10, 20, 50, and 100 μM) for 1 h and stimulated by PMA (10 ng mL⁻¹) for 24 h. The relative amount of MMP-2 and MMP-9 was quantified by densitometry measurement (ImageJ). The results presented are the means ± S.D. of a triplicate experiment. ^#P < 0.001, P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, PMA stimulation.

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