1-(5-Bromo-2-hydroxy-4-methoxyphenyl)ethanone [SE1] Inhibits MMP-9 Expression by Regulating NF-B and MAPKs Signaling Pathways in HT1080 Human Fibrosarcoma Cells

Figure 5

Cells were treated with 50 and 100 μM SE1 for 1 h and then stimulated with PMA (10 ng mL⁻¹) for 24 h. (a) Nuclear translocation of NF-κBp65 was monitored by an overlay of blue DAPI staining with green p65 immunofluorescence. (b) p-65 nuclear localization was measured. Untreated control was used as a loading control. The results presented are the means ± S.D. of a triplicate experiment. ^#P < 0.001 and ^∗∗∗P < 0.001, PMA stimulation.

(a)

(b)