Anti-Inflammatory and Antioxidant Properties of the Extract, Tiliroside, and Patuletin 3-O-β-D-Glucopyranoside from Pfaffia townsendii (Amaranthaceae)

Table 2

Total phenol content and antioxidant capacity by the DPPH and ORAC assays of crude extract, phases of partition, and flavonoids isolated from P. townsendii.

Sample; Ethanol extract	Phenol content; (mg of GAE/g)	DPPH assay,; (µg/mL)	ORAC assay; (µmol of TE/g)
Hexane crude extract	< 1.13	> 200	13.0 (5.0)
Ethanol crude extract	3.3 (6.3)	62.6 (10.0)	1555 (10.5)
Hexane phase	1.0 (3.5)	> 200	2461 (9.7)
Dichloromethane phase	2.5 (7.9)	45.6 (6.3)	5641 (4.9)
Hydroalcoholic phase	2.0 (2.8)	31.9 (7.7)	4581 (7.7)
Patuletin 3-O-β-D-glucopyranoside	-	4.9 (2.1)	4.2 (6.9)
Tiliroside	-	83.2 (3.2)	0.8 (3.4)
Mixture of patuletin 3-O-β-D-glucopyranoside + Tiliroside		3.7 (2.5)	4.8 (11.4)
Quercetin	-	8.3 (2.1)	5.6 (0.9)
Caffeic acid	-	11.2 (2.4)	2.9 (2.0)
Trolox	-	2.8 (1.8)	-

Mean value (%RSD, relative standard deviation) of triplicate assays. Total phenolics data expressed as mg of gallic acid equivalents per g (mg of GAE/g). DPPH assay data expressed as SC₅₀ (concentration that inhibited 50% of the DPPH radical) in μg per mL. ORAC data expressed as μmol of Trolox equivalents per g (µmol of TE/g). ORAC data expressed as relative Trolox equivalent, mean (%RSD, relative standard deviation) of triplicate assays. Experimental positive controls: not evaluated.