Drug-Repositioning Approach for the Discovery of Anti-Influenza Virus Activity of Japanese Herbal (Kampo) Medicines In Vitro: Potent High Activity of Daio-Kanzo-To

Figure 2

Anti-influenza virus activity of commercially available Daio-kanzo-to and Mao-to. (a) and (b) show representative data from three independent experiments. ((a), (b)) Cytotoxicity and anti-influenza virus activity of Daio-kanzo-to and Mao-to. MDCK cells in a 96-well culture plate, treated with serial dilutions of Daio-kanzo-to (TJ-84) or Mao-to (EK-27), were incubated at 37°C for 48 h with or without A/WSN/33 virus. Oseltamivir (13.6 μg/mL) was used as a control. The cells were stained by CV. ((c), (d)) Culture supernatants and CV stained results in (a) and (b) were used for determination of virus titer and relative OD values, respectively. For determination of virus titer, culture supernatants were collected before fixation and the virus titers (gray rectangles) were determined by the TCID₅₀ method. Relative OD values in the presence (closed circles) or absence (open circles) of virus was calculated with reference to uninfected untreated control. Average values from duplicate measurements are shown.

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