Hydroalcoholic Extract and Ethyl Acetate Fraction of Bixa orellana Leaves Decrease the Inflammatory Response to Mycobacterium abscessus Subsp. massiliense

Figure 3

Effects of Bixa orellana extracts (BoHE and BoEA) on cell migration to peritoneal fluid after induction of peritonitis by heat killed M. abscessus (Mabs). Saline: animals treated with 0.9% saline solution (1 mL/kg) without Mabs inoculation (negative control); Mabs: animals treated with 0.9% saline solution (1 mL/kg) and inoculated with Mabs; Dexa: animals treated with 5 mg/kg dexamethasone and inoculated with Mabs; BoHE 50 mg/kg: animals treated with 50 mg/kg BoHE and inoculated with Mabs; BoHE 150 mg/kg: animals treated with 150 mg/kg BoHE and inoculated with Mabs; BoEA 50 mg/kg: animals treated with 50 mg/kg BoEA and inoculated with Mabs; BoEA 150 mg/kg: animals treated with 150 mg/kg BoEA and inoculated with Mabs. (a) Total leukocytes 4h after induction of peritonitis. (b) Total leukocytes 24h after induction of peritonitis. (c) Polymorphonuclear cells (PMN) 4h after induction of peritonitis. (d) Polymorphonuclear cells (PMN) 24h after induction of peritonitis. (e) Mononuclear cells (MN) 4h after induction of peritonitis. (f) Mononuclear cells (MN) 24h after induction of peritonitis. p < 0.001 compared with Mabs controls. ^#p < 0.001 compared with saline controls.

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