Hydroalcoholic Extract and Ethyl Acetate Fraction of Bixa orellana Leaves Decrease the Inflammatory Response to Mycobacterium abscessus Subsp. massiliense

Figure 4

Evaluation of the toxicity of Bixa orellana extracts (BoHE and BoEA) in C57BL/6 mice 4 h after induction of peritonitis. Saline: animals treated with 0.9% saline solution (1 mL/kg) without Mabs inoculation (negative control); Mabs: animals treated with 0.9% saline solution (1 mL/kg) and inoculated with Mabs; Dexa: animals treated with 5 mg/kg dexamethasone and inoculated with Mabs; BoHE 50 mg/kg: animals treated with 50 mg/kg BoHE and inoculated with Mabs; BoHE 150 mg/kg: animals treated with 150 mg/kg BoHE and inoculated with Mabs; BoEA 50 mg/kg: animals treated with 50 mg/kg BoEA and inoculated with Mabs; BoEA 150 mg/kg: animals treated with 150 mg/kg BoEA and inoculated with Mabs. Column (A): esophageal/stomach transition area with no significant histological changes. Column (B): liver histology has a normal appearance. Column (C): kidney histology has a normal appearance. H&E stain, ×100.