Sheng-Jiang Powder Ameliorates High Fat Diet Induced Nonalcoholic Fatty Liver Disease via Inhibiting Activation of Akt/mTOR/S6 Pathway in Rats

Figure 2

The macroscopic and microscopic appearance as well as the pathological images of liver among the three experimental groups. (a) The macroscopic appearance of liver. The fresh liver in NG showed a fine texture, dark red color and sharp edge in appearance (left), while the fatty liver appeared slightly swollen with a greasy texture, and the color turned yellow, the edge became dull in HFG (middle), and the liver tissues in SG appeared close to normal (right). (b) The microscopic appearance of liver among the three experimental groups. To better exhibit manifestation of liver in ultrastructure, we exhibited the ultrastructure images with three magnifications: 1k, 1.5k, and 2k. Plenty of lipid droplets (blue arrow) were observed by transmission electron microscope in HFG and the ultrastructure of liver cells showed apparent changes with endoplasmic reticulum expansion (red arrow), mitochondrial contraction (yellow arrow), and cell matrix solidification (green arrow). (c) Oil red O staining of frozen liver sections. The frozen liver sections showed obvious lipid droplets accumulation in HFG, while it was much better in SG. (d) Pathological images of liver. Hematoxylin-eosin counterstain. Histological images are presented with original magnification 200×. Liver in HFG exhibited enlarged hepatocytes (orange arrow), extensive vacuolization (purple arrow), and inflammatory cells infiltration (black arrow), and these changes were ameliorated in SG.

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