Development of a HPLC-MS/MS Method to Determine 11 Bioactive Compounds in Tongmai Yangxin Pill and Application to a Pharmacokinetic Study in Rats

Table 4

Extraction recoveries and matrix effects of the analytes (n=6).

Compounds	Spiked concentration (ng/mL)	Extraction recovery (%)	RSD (%)	Matrix effect (%)	RSD (%)
rhein	1	96.2±7.4	7.7	104.1±5.6	5.4
	10	99.3±3.9	3.9	100.1±2.7	2.7
	100	101.4±5.2	5.1	92.6±3.7	4.0
emodin	2	98.8±3.4	3.5	107.7±2.6	2.4
	20	96.2±2.5	2.6	103.6±0.8	0.8
	200	86.1±4.2	4.9	104.8±2.9	2.8
stilbene glycoside	2	98.9±1.9	1.9	100.7±2.2	2.2
	20	99.3±4.0	4.0	94.6±2.2	2.3
	200	96.7±5.6	5.8	98.7±0.8	0.9
gallic acid	2	99.1±3.1	3.2	110.4±5.8	5.2
	20	100.9±2.5	2.5	98.3±2.0	2.0
	200	85.2±3.0	3.5	108.1±1.3	1.2
ononin	2	91.8±2.7	2.9	98.6±2.8	2.8
	20	89.0±3.8	4.3	89.9±0.7	0.8
	200	93.1±5.2	5.6	91.7±0.9	1.0
verbascoside	1	93.1±4.0	4.3	103.6±3.3	3.1
	10	97.0±4.7	4.8	100.0±2.1	2.1
	100	92.5±6.1	6.6	98.9±2.3	2.4
liquiritin	10	105.7±4.5	4.3	98.8±1.2	1.2
	100	109.1±4.0	3.6	100.1±6.4	6.4
	1000	99.5±7.6	7.7	95.2±5.5	5.8
schisandrin	2	98.8±4.1	4.2	96.8±2.6	2.7
	20	106.3±1.1	1.0	93.0±1.7	1.8
	200	101.0±6.0	5.9	90.4±0.7	0.8
liquiritigenin	1	99.7±3.5	3.6	113.4±2.8	2.5
	10	99.5±2.7	2.7	89.2±1.3	1.5
	100	96.3±3.4	3.5	99.8±1.8	1.8
glycyrrhizic acid	20	100.9±7.5	7.4	101.6±6.8	6.7
	200	97.5±2.1	2.1	102.5±1.3	1.3
	2000	87.6±1.6	1.8	103.7±2.1	2.0
isoliquiritigenin	1	98.0±5.5	5.6	105.73±2.2	2.1
	10	96.5±2.0	2.1	98.0±3.6	3.6
	100	85.9±5.7	6.7	97.3±3.1	3.2