Research Article
Development of a HPLC-MS/MS Method to Determine 11 Bioactive Compounds in Tongmai Yangxin Pill and Application to a Pharmacokinetic Study in Rats
Table 5
Stability of all analytes in rat plasma (n=6).
| Compounds | Spiked Concentration (ng/mL) | room temperature for 6 h | three freeze-thaw cycles | auto-sampler for 12 h | -70°C for 14 days | Measured Concentration (ng mL−1) | RSD (%) | Measured Concentration (ng mL−1) | RSD (%) | Measured Concentration (ng mL−1) | RSD (%) | Measured Concentration (ng mL−1) | RSD (%) |
| rhein | 1 | 0.97±0.02 | 2.0 | 0.98±0.03 | 2.8 | 0.97±0.01 | 0.8 | 0.95±0.05 | 5.4 | 10 | 10.23±0.04 | 0.4 | 8.89±0.08 | 0.9 | 9.86±0.09 | 0.9 | 8.71±0.11 | 1.3 | 100 | 101.97±1.07 | 1.1 | 86.7±1.33 | 1.5 | 97.32±1.05 | 1.1 | 85.95±0.39 | 0.5 | emodin | 2 | 1.95±0.05 | 2.3 | 2.00±0.02 | 0.9 | 1.93±0.02 | 0.8 | 1.86±0.03 | 1.6 | 20 | 21.14±0.99 | 4.7 | 19.21±0.33 | 1.7 | 19.88±1.81 | 9.1 | 18.79±0.18 | 0.9 | 200 | 207.49±1.27 | 0.6 | 197.74±5.65 | 2.9 | 200.50±11.66 | 5.8 | 185.45±0.98 | 0.5 | stilbene glycoside | 2 | 1.82±0.01 | 0.8 | 2.00±0.03 | 1.6 | 1.89±0.00 | 0.1 | 1.99±0.01 | 0.3 | 20 | 20.88±1.19 | 5.7 | 19.87±1.43 | 7.2 | 19.00±0.64 | 3.4 | 20.01±0.31 | 1.6 | 200 | 202.79±12.80 | 6.3 | 200.45±2.10 | 1.1 | 182.40±1.23 | 0.7 | 203.34±1.39 | 0.7 | gallic acid | 2 | 1.97±0.03 | 1.5 | 1.93±0.04 | 1.8 | 2.03±0.05 | 2.3 | 1.99±0.04 | 2.0 | 20 | 20.77±1.09 | 5.3 | 20.08±0.51 | 2.5 | 19.27±0.35 | 1.8 | 19.26±0.22 | 1.2 | 200 | 205.97±2.14 | 1.0 | 196.77±1.09 | 0.6 | 184.54±2.46 | 1.3 | 199.19±1.80 | 0.9 | ononin | 2 | 2.02±0.03 | 1.4 | 1.85±0.01 | 0.7 | 1.83±0.01 | 0.4 | 1.95±0.01 | 0.5 | 20 | 20.57±0.26 | 1.2 | 18.71±0.26 | 1.4 | 18.84±0.62 | 3.3 | 18.51±0.15 | 0.8 | 200 | 216.32±0.91 | 0.4 | 191.28±2.36 | 1.2 | 195.34±4.30 | 2.2 | 200.23±3.89 | 1.9 | verbascoside | 1 | 0.92±0.01 | 1.0 | 1.09±0.01 | 0.9 | 1.05±0.02 | 1.8 | 1.01±0.02 | 2.3 | 10 | 9.57±0.21 | 2.2 | 10.17±0.90 | 8.9 | 9.50±0.04 | 0.5 | 9.81±0.22 | 2.2 | 100 | 103.95±1.33 | 1.3 | 108.42±1.17 | 1.1 | 93.52±0.27 | 0.3 | 107.40±1.47 | 1.4 | liquiritin | 10 | 9.85±0.09 | 1.0 | 9.26±0.16 | 1.7 | 10.26±0.11 | 1.1 | 10.04±0.12 | 1.2 | 100 | 108.97±1.51 | 1.4 | 109.67±1.86 | 1.7 | 108.02±0.66 | 0.6 | 105.05±1.73 | 1.7 | 1000 | 1076.07±10.27 | 1.0 | 952.89±11.22 | 1.2 | 869.12±14.52 | 1.7 | 1021.69±19.98 | 2.0 | schisandrin | 2 | 2.00±0.04 | 1.8 | 1.71±0.01 | 0.6 | 1.83±0.02 | 1.1 | 1.77±0.03 | 1.7 | 20 | 21.15±0.50 | 2.4 | 18.28±0.23 | 1.2 | 18.35±0.09 | 0.5 | 17.86±0.20 | 1.1 | 200 | 194.61±1.13 | 0.6 | 189.32±1.74 | 0.9 | 196.02±2.10 | 1.1 | 183.83±1.00 | 0.6 | liquiritigenin | 1 | 0.87±0.01 | 0.9 | 0.89±0.02 | 1.8 | 0.87±0.01 | 1.1 | 0.88±0.02 | 2.1 | 10 | 10.82±0.58 | 5.3 | 8.99±0.07 | 0.8 | 8.63±0.23 | 2.7 | 9.26±0.08 | 0.9 | 100 | 112.69±1.30 | 1.2 | 92.59±0.63 | 0.7 | 92.65±0.68 | 0.7 | 91.88±0.86 | 0.9 | glycyrrhizic acid | 20 | 20.49±0.79 | 3.9 | 19.30±0.20 | 1.0 | 19.25±0.34 | 1.8 | 18.77±0.09 | 0.5 | 200 | 203.22±3.32 | 1.6 | 188.63±3.57 | 1.9 | 200.96±3.93 | 2.0 | 200.33±2.98 | 1.5 | 2000 | 2099.10±8.92 | 0.4 | 1869.00±42.55 | 2.3 | 1884.66±9.26 | 0.5 | 1834.54±29.34 | 1.6 | isoliquiritigenin | 1 | 0.99±0.01 | 1.5 | 0.89±0.02 | 2.4 | 1.00±0.02 | 1.7 | 0.89±0.01 | 1.4 | 10 | 10.86±0.60 | 5.5 | 8.69±0.14 | 1.6 | 9.60±0.04 | 0.4 | 8.62±0.04 | 0.4 | 100 | 106.11±0.78 | 0.7 | 100.58±0.91 | 0.9 | 97.47±1.99 | 2.0 | 100.74±2.95 | 2.9 |
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