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Evidence-Based Complementary and Alternative Medicine
Volume 2018, Article ID 6509650, 5 pages
https://doi.org/10.1155/2018/6509650
Research Article

Protective Effect of Electroacupuncture at Zusanli on Myocardial Injury in Septic Rats

1Department of Rehabilitation of the 309th Hospital of People’s Liberation Army, Beijing 100091, China
2Department of Acupuncture and Moxibustion, People’s Liberation Army General Hospital, Beijing 100853, China
3Laboratory of Shock and Organ Dysfunction, Burns Institute, First Affiliated Hospital of PLA General Hospital, Beijing 100037, China
4Research Unit for Complementary and Integrative Laser Medicine, Research Unit of Biomedical Engineering in Anesthesia and Intensive Care Medicine, and TCM Research Center Graz, Medical University of Graz, 8036 Graz, Austria

Correspondence should be addressed to Xian Shi; nc.evil@xs103 and Gerhard Litscher; ta.zarginudem@rehcstil.drahreg

Received 15 April 2018; Accepted 9 September 2018; Published 8 October 2018

Academic Editor: Thomas Lundeberg

Copyright © 2018 Lijian Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The protective effect of electroacupuncture (EA) at Zusanli points (ST36) on myocardial injury in a model of sepsis was observed. Forty-eight male Sprague-Daley rats were subjected to sepsis by cecal ligation and puncture (CLP) and randomly divided into 4 groups (n=12; A: sepsis + EA; B: sepsis + sham acupuncture; C: sepsis + vagotomy; D: sepsis + vagotomy + EA). Bilateral points were stimulated (2mA, 2-100 Hz) for 1 hour. Abdominal vagotomy was performed in groups C and D. At 6h after CLP, the plasma activity of creatine kinase-MB (CK-MB) was determined. A part of cardiac muscle was harvested for evaluating levels of tumor necrosis factor (TNF-α), nitric oxide (NO), myeloperoxidase (MPO), and the rate of water content. The activities of CK-MB, TNF-α, NO, and MPO and the rate of water content in group A were significantly lower than those of the other groups 6h after CLP. EA after vagotomy showed less anti-inflammatory and protective effects. The results indicated that EA obviously reduced the increased levels of the proinflammatory factors at 6h after CLP, and vagotomy could weaken or eliminate the effects of EA. Cholinergic anti-inflammatory pathway is one of the main mechanisms of cardioprotective effect of EA.