ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFR/NF-κB Activities of HeLa Human Cervical Cancer Cells

<div>Evaluation of Manilkara zapota leaf methanol extract-induced apoptotic cell death in (a) untreated HeLa cells and HeLa cells treated with (b) 12 μg/mL, (c) 24 μg/mL, and (d) 48 μg/mL of Manilkara zapota leaf methanol extract for 72 h. (e) Assessment of apoptotic cell death treated with Manilkara zapota leaf methanol extract was determined using Annexin V-FITC and propidium iodide (PI) staining assay using flow cytometry. Values are reported as mean ± SD (n = 3). Value with different superscript letter indicates significant difference between groups by Tukey’s test (P &lt; 0.05).</div>

Evidence-Based Complementary and Alternative Medicine

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Figure 5

Figure 5: ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFR/NF-κB Activities of HeLa Human Cervical Cancer Cells

Figure 5 | ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFR/NF-κB Activities of HeLa Human Cervical Cancer Cells 