Genotoxic and Chemopreventive Effects of Vochysia divergens Leaves (Pantanal, Brazil)

Table 2

Frequencies of micronucleated polychromatic erythrocytes (MNPCEs) and PCE/PCE+NCE ratio in Swiss mouse bone marrow treated with VdE and/or MMS and their respective controls.

Treatment; (mg/kg b.w.)	MNPCEs	PCE/PCE + NCE
Control	6.33 ± 1.75	0.63 ± 0.13
DMSO	6.33 ± 2.34	0.63 ± 0.08
500	4.33 ± 1.96	0,.66 ± 0.09
1000	4.16 ± 1.47	0.72 ± 0.08
2000	7.83 ± 2.78	0.60 ± 0.05
MMS	41.50 ± 9.13	0.61 ± 0.10
DMSO + MMS	48.00 ± 9.40	0.61 ± 0.06
15 + MMS	22.83 ± 2.48	0.63 ± 0.05
30 + MMS	42.60 ± 6.43	0.55 ± 0.12
60 + MMS	47.00 ± 5.17	0.62 ± 0.05

VdE: ethanolic extract of Vochysia divergens leaves; PCE: polychromatic erythrocytes; NCE: normochromatic erythrocytes; DMSO: dimethyl sulfoxide (5%); MMS: methyl methanesulfonate (40 mg/kg b.w.). Values are the mean ± standard deviation. A total of 400 erythrocytes were analyzed per animal, corresponding to 2,400 cells per treatment. A total of 2,000 PCEs were analyzed per animal, corresponding to 12,000 cells per treatment. Significantly different from control (P < 0.05). Significantly different from the MMS group (P < 0.05).