Total Flavonoids from Radix Glycyrrhiza Exert Anti-Inflammatory and Antitumorigenic Effects by Inactivating iNOS Signaling Pathways

Figure 4

The effect on TFRG on tumor growth in vivo. (a) Tumors were collected and weighted. Quantification of tumor weight in vehicle, TFRG (20, 100 mg/kg/d) with indicated concentrations and L-NIL (10 mg/kg/d), the positive control groups. , versus model group. (b) The tumor tissues with/without TFRG and L-NIL were excised quickly and stored in −80°C. Detection content of 3-NT was carried out according to the kit protocol and data were quantified with mg protein of each sample. (c) The tumors of mice treated with/without TFRG and L-NIL were collected and subjected to Western blotting to detect iNOS, phosphor-JAK2/total JAK2, and phosphor-STAT3/total STAT3. M: model group; TFRG 20, TFRG 100: TFRG at 20, 100 mg/kg/d; L-NIL10: L-NIL at 10 mg/kg/d dosage.

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