(a)
(b)
(c)
Figure 2: Suppression in the expression of proinflammatory mediators and cytokines by the Rg3-RGE extract. (a–b) RAW 264.7 cells were preincubated with Rg3-RGE for 30 min and then stimulated with LPS for 18 h. Later the expression of proinflammatory mediators and cytokines were determined by RT-PCR. Moreover in (c), proteins were extracted from RAW 264.7 cells and the level of iNOS and COX-2 were measured by western blotting. Values in the bar graphs are means ± SEM of three independent experiments. p < 0.001 are considered significant compared to the LPS-only group.