Figure 5: Rg3-RGE-induced signal transduction through the NF-κB pathway and its inhibitor in vitro and in vivo. RAW 264.7 cells were preincubated with Rg3-RGE for 30 min and then stimulated with LPS for 18 h. Nuclear and cytosolic proteins were extracted from cells using the NE-PER® Nuclear and Cytosolic Extraction Reagents. β-actin was used as an internal control. Image is representative of three independent experiments. (a, b) Phosphorylation of NF-κB pathway factors in RAW cells. (c, d) Inhibition of phosphorylation of p- NF-κB in testis and lungs tissue. (e) Expression of BAY11-7085 (NF-κB inhibitor). Values in bar graphs are means ± SEM of three independent experiments. p < 0.001 is considered significant compared to the LPS-only group.