Evidence-Based Complementary and Alternative Medicine / 2018 / Article / Fig 1

Research Article

Anti-Inflammatory Potential of Carpomitra costata Ethanolic Extracts via Inhibition of NF-κB and AP-1 Activation in LPS-Stimulated RAW264.7 Macrophages

Figure 1

Effect of CCE on LPS-induced NO and PGE2 production in RAW264.7 macrophages. (a) RAW264.7 cells were pretreated with the indicated concentrations of CCE (30–100 μg/mL) for 24 h. Cell viability was assessed using the CCK-8 assay and the results are expressed as the percentage of surviving cells relative to control cells (DMSO-treated). (b and c) The cells were pretreated with CCE (30–100 μg/mL) for 1 h and stimulated with LPS (100 ng/mL) for 24 h. Levels of NO and PGE2 in the supernatant were detected using the Griess reaction assay (b) and ELISA (c). Each value indicates the mean ± SEM and is representative of the results obtained from three independent experiments. versus control group; versus LPS-stimulated group. DEX: dexamethasone.