Propolin C Inhibited Migration and Invasion via Suppression of EGFR-Mediated Epithelial-to-Mesenchymal Transition in Human Lung Cancer Cells
Effects of propolin C on PI3K/Akt and ERK-mediated EMT marker expressions in HCC827 lung cancer cells. HCC827 cells were treated with propolin C (2.5, 5, 7.5, and 10 μM) for 24 h. After treatment, the expressions of (a) E-cadherin, vimentin, slug, snail, and β-actin and (b) phospho-ERK, ERK, phospho-Akt, and Akt were analyzed by Western blot as described in Materials and Methods. Significant difference was observed from the control group ( < 0.05). (c) HCC827 cells were pretreated with LY294002 (LY, 10 μM) or PD98059 (PD, 10 μM) for 30 min and then incubated with or without propolin C (PPC, 10 μM) for 24 h. After incubation, cells were harvested and Western blot analyses were used to detect E-cadherin, vimentin, snail, and β-actin expressions. Data were shown as mean ± SD (). Different uppercase letters (A−D) indicate statistical differences among group (), and the same letter showed no difference ().