Guzhi Zengsheng Zhitongwan, a Traditional Chinese Medicinal Formulation, Stimulates Chondrocyte Proliferation through Control of Multiple Genes Involved in Chondrocyte Proliferation and Differentiation

Figure 1

Cell viability assay in primary chondrocytes treated with GZZSZTW and DAE. The CCK-8 kit was used to detect chondrocyte proliferation following treatment with GZZSZTW and DAE at increasing concentrations (0, 0.2, 0.4, 0.6, 0.8, and 1.0 mg/ml) for 24 h, respectively. Cell viabilities were normalized and calculated relative to the untreated group (0 mg/ml). Data are presented as the mean and standard deviation for technical triplicates in an experiment representative of several other independent experiments. (Δ) represents p <0.01 and (ΔΔ) represents p <0.001, computed by the t-test for the difference in cell viability in response to GZZSZTW and DAE treatment, respectively.