The Antiapoptosis Effect of Geum japonicum Thunb. var. chinense Extracts on Cerebral Ischemia Reperfusion Injury via PI3K/Akt Pathway

Figure 5

Treatment of different concentration of GJ extracts 7 days prior to MCAO increased Bcl-2 expression and decreased expression of Bax and cleaved caspase-3 by immunohistochemistry. We analyzed the levels of Bcl-2, Bax, and cleaved caspase-3 in brain sections by immunohistochemistry staining. (a) Representative pictures of Bcl-2, Bax, and cleaved caspase-3 positive cells in the tissue. (b, c, and d) As demonstrated in the bar graphs, immunohistochemistry staining results of dates are demonstrated as the mean ± SEM. <0.01 versus sham group; p< 0.01 versus I/R group. Scale bar 100 μm.

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