Dietary 1.5% TU-100 Alters Fecal Microbiota and the Effect is Reversible. PCoA plots of unweighted and weighted UniFrac distances were generated for female ((a), (c), and (e)) and male ((b), (d), and (f)) samples at weeks 0 ((a) and (b)), 12 ((c) and (d)), and 24 ((e) and (f)). Red squares indicate group 1 (fed AIN76A diet for 24 weeks), blue triangles group 2 (fed TU-100 for 24 weeks), and orange circles group 3 (fed TU-100 for 12 weeks and then AIN-76A for 12 weeks). ((a) and (b)) At week 0, the microbiota among mice in each group appeared to be randomly distributed in each sex. ((c) and (d)) At week 12, plots for mice in group 2 and group 3 after 12-week consumption of 1.5% TU-100 clustered together and separately from group 1 without TU-100 in unweighted UniFrac distances for both sexes. ((e) and (f)) At week 24, the plots of group 3 appeared to be between groups 1 and 2 in PCoA of unweighted UniFrac distances for both sexes.