Alleviating the Intestinal Absorption of Rhein in Rhubarb through Herb Compatibility in Tiaowei Chengqi Tang in Caco-2 Cells

Figure 3

Effects of inhibitors on permeability (a) and uptake (b) of rhein and effects of RE, PE, RPE, and TWCQT on uptake (c) of rhein by Caco-2 cells. B-A: the basolateral to apical side; A-B: the apical to basolateral side. Verapamil: 50 μmol/ml; MK-571: 50 μmol/ml; Ko143: 10 μmol/ml; rhein: 27.3 μg/ml rhein; RE + rhein: 1 mg/ml rhubarb extract + 18.69 μg/ml rhein; PE + rhein: 1 mg/ml prepared licorice extract + 27.3 μg/ml rhein; RPE: 1 mg/ml rhubarb + prepared liquorice extract; TWCQT: 1 mg/ml Tiaowei Chengqi Tang. Data are represented by the mean ± SD from three replicates. < 0.05; < 0.01 compared with 27.3 μg/ml rhein.

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