Establishing a Cell-Based High-Content Screening Assay for TCM Compounds with Anti-Renal Fibrosis Effects
Optimization of high-content screening assay parameters. (a) Effect of serum on the expression of α-SMA in NRK49F cells with or without stimulation with TGF-β1 for 72 h by immunofluorescence. (b) Comparison of α-SMA expression in NRK49F cells with or without TGF-β1 stimulation for 72 h when treated with different serum concentrations. Comparison of α-SMA expression in NRK49F cells with or without TGF-β1 stimulation at different incubation times (c) and different concentrations (d). Means ± SD, ns: P > 0.05, : P < 0.05, : P < 0.01, : P < 0.001.