Establishing a Cell-Based High-Content Screening Assay for TCM Compounds with Anti-Renal Fibrosis Effects

Figure 5

(a) Representative compound, Hydroxysafflor Yellow A, images of high-content screening analysis for the validation of compounds with antifibrosis effects. The effect of compounds on the expression of α-SMA (green) and collagen III (red) of TGF-β1-treated NRK49F cells was obtained. Nuclei were identified by Hoechst 33342 staining (blue). (b). Effect of TCM compounds on the expression of α-SMA and collagen III in TGF-β1-treated NRK49F cells. (“1” indicates obvious inhibitory effects; “-1” indicates no obvious inhibitory effects; “0” indicates that although there is no statistical difference, there is a decreasing trend.)

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