Ethanol Extract of Cudrania tricuspidata Leaf Ameliorates Hyperuricemia in Mice via Inhibition of Hepatic and Serum Xanthine Oxidase Activity

Table 1

Analytical conditions of the GC-MS and preparative HPLC methods.

Parameter	Condition
	GC-MS
Column	Agilent HP-5MS fused silica capillary column
	(30 m × 0.25 mm i.d., 0.25 μm film thickness)
Carrier	Helium
Split	1 : 5
Injection volume	1 μL
MS source	230°C
MS quad	150°C
Analytical temperature		Rate	Value	Hold time
	Initial		65°C	10 min
	Ramp	10°C/min	300°C	22 min
	Total	55.5 min
Thermal aux	300°C
Electron ionization	70 ev
Mass range	50–550 amu
Scan method	Full scan
	Preparative HPLC
Column	OP C18-51002510 (250×10.0 mm, 5 μm)
Flow rate	3 mL/min
Injection volumn	1000 μL
UV detection	250 nm
Run time	25 min
Gradient flow	Time (min)	ACN (v/v%)	0.2% phosphoric acid (v/v%)
	0	10	90
	5	10	90
	18	50	50
	20	100	0
	21	10	90
	25	10	90