Evidence-Based Complementary and Alternative Medicine / 2018 / Article / Fig 6

Research Article

JYYS Granule Mitigates Renal Injury in Clinic and in Spontaneously Hypertensive Rats by Inhibiting NF-B Signaling-Mediated Microinflammation

Figure 6

JYYS granule inhibited expression of microinflammatory genes and ameliorates NF-κB mediated renal inflammation in SHR. (a~d) Real-time PCR was used to analyze the mRNA expression of microinflammatory genes, including IL-6 (a), TNF-α (b), ICAM-1 (c), and MCP-1 (d); results are showed as mean ± SD, n=10, #P <0.05 versus WKY group, Δ<0.05 versus SHR group. (e) Real-time PCR was conducted to analyze IκB mRNA expression; data are expressed as mean ± SD, n=10, #P <0.05 versus WKY group, Δ<0.05 versus SHR group. ((f) and (g)) Immunohistochemistry (IHC) was performed to detect the effects of JYYS granule on renal IκB protein expression and the semiquantification of these genes was based on the fluorescence intensity (f); data are expressed as mean ± SD, n=10, #P <0.05 versus WKY group, Δ<0.05 versus SHR group (g). ((h) and (i)) Western blot was conducted to evaluate the effects of JYYS granule on renal IκB protein expression (h); data are expressed as mean ± SD, n=10, #P <0.05 versus WKY group, Δ<0.05 versus SHR group (i). ((j) and (k)) Immunohistochemistry (IHC) was carried out to examine the effects of JYYS granule on renal IκB expression and the semiquantification of these genes was based on the intensity. Data are expressed as mean ± SD, n=10, #P <0.05 versus WKY group, Δ<0.05 versus SHR group.
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