Figure 4: UroA attenuates high glucose- (HG-) induced A7r5 cell proliferation by suppressing Akt/β-catenin signaling. (a) The effect of UroA on high glucose- (HG-) induced A7r5 cell proliferation. Cells were seeded in 96 well and preincubated with HG (30 mM) for 12 h. The cells were treated by UroA (5–40 μM). CCK-8 assay was performed to determine cell viability as described in Materials and Methods. (b) Western blotting analysis for the protein expressions of phosphorylated-Akt (Thr308) (60 kD) and β-catenin (92 kD) in HG-incubated A7r5 cells. (C) Histograms show phosphorylated-Akt (Thr308) and β-catenin protein levels quantified by western blot optical analysis. (c) The effect of UroA on the mRNA expressions of c-Myc and cyclin D1 was quantified by qPCR in HG-induced VSMC proliferating model. Quantitative data are mean ± SD, . versus MOCK; versus HG group.