Research Article
Emblic Leafflower (Phyllanthus emblica L.) Fruits Ameliorate Vascular Smooth Muscle Cell Dysfunction in Hyperglycemia: An Underlying Mechanism Involved in Ellagitannin Metabolite Urolithin A
Figure 5
UroA suppresses LiCl-induced activation of Wnt/β-catenin signaling in A7r5 cells. (a, b) Cells were preincubated with LiCl (20 mM) for 3 h. Then the cells were further treated with UroA for 48 h. Western blotting analysis was applied for quantifying the protein expression of β-catenin (92 kD) in LiCl-pretreated A7r5 cells. (c) UroA suppressed Tcf/Lef-mediated transcriptional activity in A7r5 cells. Cells were transiently cotransfected with TopFlash or FopFlash and Renilla reporter plasmid with Lipofectamine 3000. After posttransfection, cells were treated as described in Materials and Methods. Luciferase values were measured using a dual-luciferase reporter assay system. Quantitative data are mean ± SD, . , versus MOCK; and versus LiCl20 group.
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