Simultaneous Determination of Columbianadin and Its Metabolite Columbianetin in Rat Plasma by LC-MS/MS: Application to Pharmacokinetics of Columbianadin after Oral Administration

Table 3

Recoveries, matrix effects, stability and intraday and interday accuracy, and precision of the columbianetin and columbianadin (n = 6).

Compound	Concentration; (ng/mL)	Recovery		Matrix effect		Freeze-thaw three cycles		-20°C for 2 weeks		Autosampler for 24 h		Intraday		Interday
		Mean (%)	RSD; (%)	Mean (%)	RSD; (%)	Remains (%)	RSD; (%)	Remains (%)	RSD; (%)	Remains (%)	RSD; (%)	Accuracy (%)	RSD; (%)	Accuracy; (%)	RSD; (%)
Columbianetin	0.5	89.2; 89.7; 88.2	2.03	96.0; 93.1; 95.6	2.12	105	5.51	112	5.72	96.8	1.82	101	2.61	96.4	7.02
	4; 20		2.43; 4.83		3.53; 5.52	101; 95	1.87; 4.36	107; 93.8	5.15; 5.79	88.7; 88.8	4.6; 2.88	98.3; 94.9	2.78; 11.7	97.9; 97.4	10.0; 10.9
Columbianadin	0.5	92.8	2.88	93.8	4.18	99.4	2.13	98.6	2.27	102	6.72	94.1	1.95	98.7	4.08
	4	94.2	1.79	90.7	4.05	106	5.01	101	2.66	101	0.8	98.3	3.63	100	2.79
	20	95.9	5.44	96.1	10.0	93.2	6.21	101	4.63	106	6.05	94.4	6.95	99.2	7.03