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Evidence-Based Complementary and Alternative Medicine
Volume 2018, Article ID 8617314, 13 pages
Research Article

Total Flavonoids from Carya cathayensis Sarg. Leaves Alleviate H9c2 Cells Hypoxia/Reoxygenation Injury via Effects on miR-21 Expression, PTEN/Akt, and the Bcl-2/Bax Pathway

1College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China
2Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou 310022, China
3College of Medical Technology, Zhejiang Chinese Medical University, Hangzhou 310053, China

Correspondence should be addressed to Bo Jin; nc.ude.umcz@obnij

Ruibin Jiang and Yan Guo contributed equally to this work.

Received 13 July 2018; Revised 29 September 2018; Accepted 21 November 2018; Published 5 December 2018

Academic Editor: Kuzhuvelil B. Harikumar

Copyright © 2018 Ruibin Jiang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study aimed to investigate whether the total flavonoids (TFs) from Carya cathayensis Sarg. leaves alleviate hypoxia/reoxygenation (H/R) injury in H9c2 cardiomyocytes and to explore potential mechanisms. H9c2 cells pretreated with TFs for 24h were exposed to H/R treatment. The results indicated that TFs significantly alleviate H/R injury, which include inhibiting apoptosis and enhancing antioxidant capacity. The protective effects of TFs resulted in higher expression of miR-21 in H/R-induced H9c2 cells than that of controls, which in turn upregulated Akt signaling activity via suppressing the expression of PTEN together with decreasing the ratio of Bax/Bcl-2, caspase3, and cleaved-caspase3 expression in H/R-induced H9c2 cells. Conversely, blocking miR-21 expression with miR-21 inhibitor effectively suppressed the protective effects of TFs against H/R-induced injury. Our study suggests that TFs can decrease cell apoptosis, which may be mediated by altering the expression of miR-21, PTEN/Akt, and Bcl/Bax.