A Chinese Herbal Preparation, Xiao-Er-An-Shen Decoction, Exerts Neuron Protection by Modulation of Differentiation and Antioxidant Activity in Cultured PC12 Cells

Figure 3

XEASD stimulates neurofilament expression of cultured PC12 cells. (a) XEASD extracts (0.3–3.0 mg/mL) were applied onto cultured PC12 cells for 48 hours. The cell lysates were collected to determine the expressions of NF68, NF160, and NF200. NGF (50 ng/mL) served as the positive control. GAPDH served as a loading control. (b) Quantification plot was shown in histograms. Values are expressed as the fold of increase to basal reading (untreated culture, set as 1). Mean ± SEM; n = 4. Statistical comparison was made with the control; < 0.05; < 0.01; < 0.001.

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