Biological Evaluation of Different Extracts of Aerial Parts of Nepeta deflersiana and Standardization of Active Extracts Using 8-Epi-7-Deoxyloganic Acid and Ursolic Acid by Validated HPTLC Method

Table 7

Robustness of the proposed HPTLC Method (n=6).

Optimization condition	1 (300 ng/band)		2 (300 ng/band)
	SD	%RSD	SD	%RSD
Mobile phase composition; (chloroform: methanol: formic acid)
(8.9:0.8:0.3)	3.49	1.17	4.19	1.71
(9:0.7:0.3)	3.51	1.18	4.17	1.70
(8.8:0.9:0.3)	3.46	1.16	4.21	1.72
Mobile phase volume; (for saturation)
(18 mL)	3.55	1.19	4.23	1.72
(20 mL)	3.51	1.18	4.20	1.71
(22 mL)	3.49	1.17	4.25	1.73
Duration of saturation
(10 min)	3.54	1.18	4.07	1.66
(20 min)	3.59	1.20	4.03	1.64
(30 min)	3.51	1.18	4.09	1.67