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Evidence-Based Complementary and Alternative Medicine
Volume 2018 (2018), Article ID 8950534, 11 pages
https://doi.org/10.1155/2018/8950534
Research Article

Subchronic and Genetic Safety Assessment of a New Medicinal Dendrobium Species: Dendrobium Taiseed Tosnobile in Rats

1Department of Pharmacy, School of Pharmacy, China Medical University, Taichung, Taiwan
2Graduate Institute of Veterinary Pathobiology, National Chung Hsing University, Taichung, Taiwan
3Taiwan Seed Improvement and Propagation Station, Council of Agriculture, Taichung, Taiwan

Correspondence should be addressed to Wen-Chuan Lin

Received 15 November 2017; Accepted 9 January 2018; Published 6 February 2018

Academic Editor: Yew-Min Tzeng

Copyright © 2018 Li-Chan Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Dendrobium Taiseed Tosnobile is a new species of herba dendrobii (Shi-Hu) that was developed by crossbreeding D. tosaense and D. nobile. Its pharmacological activity and active component have been reported, but its subchronic toxicity and genetic safety have not yet been investigated. This study assessed the 90-day oral toxicity and genetic safety of the aqueous extracts of D. Taiseed Tosnobile (DTTE) in male and female Sprague-Dawley (SD) rats. Eighty rats were divided into four groups, each consisting of ten male and ten female rats. DTTE was given orally to rats at 800, 1600, or 2400 mg/kg for 90 consecutive days, and distilled water was used for the control group. Genotoxicity studies were performed using a bacterial reverse mutation assay and in vivo mammalian cell micronucleus test in ICR mice and analyzed using flow cytometry. Throughout the study period, no abnormal changes were observed in clinical signs and body weight or on ophthalmological examinations. Additionally, no significant differences were found in urinalysis, hematology, and serum biochemistry parameters between the treatment and control groups. Necropsy and histopathological examination indicated no treatment-related changes. Based on results, the no-observed-adverse-effect level of DTTE is greater than 2400 mg/kg in SD rats.