Effect of treatments on liver histology. Photomicrographs of liver sections stained by H&E. (a) Normal rat showing normal liver architecture. (b) AIA untreated rat showing moderate portal inflammation (+++) and neutrophil infiltration (black arrow). (c, d) MTX-treated AIA rat shows distortion of liver architecture with severe portal inflammation (++++) and neutrophil infiltration (black arrow), vascular congestion (aster), and multinucleation (cross). (e) Sesame oil-treated AIA rat shows mild portal inflammation (++) with neutrophil infiltration (arrow). (f) Δ9-THC/sesame oil-treated AIA rat shows minimal portal inflammation (+) with neutrophil infiltration (arrow) and normal liver architecture.