Ethanol Extract of Lycopus lucidus Turcz. ex Benth Inhibits Metastasis by Downregulation of Runx-2 in Mouse Colon Cancer Cells

Figure 4

Effects of runt-related transcription factor-2 (Runx-2)/matrix metalloproteinase- (MMP-) 9 on the migration and invasion of CT-26 cells. (a) Effects of MMP-9 protein expressed on Runx-2 siRNA-transfected cells. (b) Effects of MMP-9 activities on Runx-2 siRNA-transfected cells. Cells were transfected for 24 h with specific Runx-2 siRNA (with fresh media without serum) and then subjected to western blot analysis or gelatin zymography. (c) Effects of migration on Runx-2 siRNA-transfected cells. (d) Densitometry of wound closure. (e) Effects of invasion on Runx-2 siRNA-transfected cells. (f) Densitometry of invasive cells. Cells were transfected for 24 h with specific Runx-2 siRNA (fresh media without serum) and then subjected to western blot analysis or gelatin zymography. Cells were transfected for 24 h with specific Runx-2 siRNA (fresh media without serum) and then analyzed for wound healing or an invasion assay. Magnification was 200×. The relative degrees of migration and invasion were quantified using ImageJ. Data are expressed as means ± standard deviation of three independent experiments. versus control group.

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