Inhibition of α-Glucosidase, Intestinal Glucose Absorption, and Antidiabetic Properties by Caralluma europaea

Figure 2

Inhibitory activity of Caralluma europaea fractions at two different doses. The essay mixture contained 0.1 mL sucrose (50 mM), 0.1 mL enzyme solution (10 UI), 1 mL of phosphate buffer (50 mM, pH=7.5), and plant fractions at two different concentrations (165 and 328 μg/mL) or acarbose (165 and 328 μg/mL). The reaction mixture was incubated at 37°C for 20 minutes. The amount of liberated glucose was measured by the glucose oxidase method, using a commercial test kit. Values are mean ± SEM (n=3) and are analyzed with one-way ANOVA followed by Dunnett’s t-test. Ns = not significant; < 0.05 versus control; < 0.01 versus control; < 0.001 versus control. HCe = hexane fraction of C. europaea; DCe = dichloromethane fraction of C. europaea; EACe= ethyl acetate fraction of C. europaea; MCe= methanol fraction of C. europaea; and ACe= aqueous fraction of C. europaea.