Acmella oleracea (L) R. K. Jansen Reproductive Toxicity in Zebrafish: An In Vivo and In Silico Assessment

Table 2

Most probable metabolites for spilanthol, with their respective chemical reactions, probability values, and predicted DL₅₀.

Metabolites	Phase type	Chemical Reaction	Probability (PR)	DL50
M8	Phase I reaction (Oxidation)	Epoxidation	0,9991	46.49 mg/kg
M18	Phase II biotransformation reaction	N- Acetylation	0,8985	26.28 mg/kg
M26	Phase I reaction; (Oxidation)	aliphatic hydroxylation	0,8072	33.14 mg/kg
M28	Phase II biotransformation reaction	Glutathionation	0,7797	327.74 mg/kg
M33	Phase I reaction; (Oxidation)	C-oxidation	0,6322	28.69 mg/kg
M35	Phase II biotransformation reaction	N-glucuronidation	0,5135	81.34 mg/kg