Mistletoe-Based Drugs Work in Synergy with Radio-Chemotherapy in the Treatment of Glioma In Vitro and In Vivo in Glioblastoma Bearing Mice

Figure 1

Induction of cell death by ML-containing drugs. (a) Cells were treated with increasing concentrations of ISCADOR Qu (ISC Qu), Aviscumine (Avi), or native ML-1 for 48 h and cell density was determined by crystal violet staining. (b) Mouse hippocampal brain slice cultures were treated for 24 h (light gray) or 48 h (dark gray) or with NMDA as a positive control of cell death induction. Dead cells were counted (n=4, SD; nd: not determined). (c) LNT-229 cells were treated with ISCADOR Qu or Aviscumine in the absence or presence of a ML-neutralizing antibody (10 µg/ml) and cell density was measured 48 h later by crystal violet staining (n=3; ±SD; Student’s t-test P < 0.05, P < 0.01, and P < 0.001; n.d.: not determined).

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