Research Article

Evaluation of Alternative Methods to Assess the Biological Properties of Propolis on Metabolic Activity and Biofilm Formation in Streptococcus mutans

Figure 1

Biofilm inhibition evaluated by crystal violet staining. S. mutans cultures were treated with different polyphenol-rich extract of propolis concentrations (0.1 to 1.6 μg·mL−1). CLX, chlorhexidine gluconate 0.2% (positive control); DMSO (vehicle control). or from the nonparametric Wilcoxon test when compared to untreated cells (100% of growth biofilm). Values are expressed as median ± SD.